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Rosie is a black widow spider with a motherlike nature. She is mostly a mother figure to the rhinoceros beetle, Dim, with whom she shares an act, and also the younger ants of the colony.
Rosie works at P.T. Flea's circus as a "lion tamer" with her partner being the rhinoceros beetle Dim. During their opening act, Rosie cracks a whip at Dim, which was actually a shoelace, as he roars ferociously. However, Rosie unintentionally lashes one of Dim's feet, making him cry in pain. Rosie consoles Dim and later attempts to put a band-aid over his "owie." Later on, Rosie and her circus troupe friends are fired after they accidentally burn P.T..