In the 1980s, a project with the goal of sequencing the human
genome started to materialise; or as they stated it: "The ultimate
goal of this initiative is to understand the human genome" and
"knowledge of the human is as necessary to the continuing progress
of medicine and other health sciences as knowledge of human anatomy
has been for the present state of medicine.".
The $3-billion project was formally founded in 1990 by the
United States Department of Energy and the U.S. National Institutes
of Health, and was expected to take 15 years. In addition to the
United States, the international consortium primarily comprised
geneticists in the United Kingdom, and also in France, Germany,
Japan, China, and India.
Due to widespread international cooperation and advances in the
field of genomics (especially in sequence analysis), as well as
major advances in computing technology, a 'rough draft' of the
genome was finished in 2000.
Source: Wikipedia
Since then, the technologies for DNA sequencing has advanced at
a rapid pace. The most common methods that are used today are often
called next-gen sequencing (NGS). In general, these methods
sequence a lot of small fragments of the wanted DNA sequence in
parallel and it is then attempted to put all these small fragments
together. The most common commercial methods are 454
pyrosequencing, Illumina sequencing and SOLiD sequencing.
In the case of SOLiD sequencing, you look on fragments (reads)
of 50 bases each. A run of the method on a long DNA sequence will
take 1-2 weeks and will generate a total of 30-50 Gigabases of
information, i.e. about 60 million short reads will be
sequenced. This means that many regions were sequenced multiple
times, and will overlap with each other.
All these reads will then have to be put together by computer
software in a process called sequence assembly. The software looks
for the overlaps between all the short reads and tries to put them
together into the real DNA sequence.
One example of how this could be done is:
>read1
AAAATTTTGGGGCCGGCCGG
>read2
GGCCGGCCGGTATATATATA
Which would produce the sequence
AAAATTTTGGGGCCGGCCGGTATATATATA when pairing the overlaps
together.
For more information about (next-gen) DNA sequencing and
assembly:
Metzker ML.
Sequencing technologies - the next generation. Nat Rev Genet. 2010
Jan; 11(1):31-46.
http://en.wikipedia.org/wiki/DNA_sequencing
Flicek P,
Birney E. Sense from sequence reads: methods for alignment and
assembly. Nat Methods. 2009 Nov; 6(11 Suppl):S6-S12.
To get the coordinates for this cache, you will do a small
sequence assembly by putting together two groups of short reads
into two sequences by looking for overlaps. The lengths of the
sequences are xxx and yyy in N 59° 20.xxx and E 018° 01.yyy.
The reads:
>N_read1
AGGGGCAAGGCTGCAAGCTCCCAGGGAGGACAGCCGGGCGGCCAAACCCT
>N_read2
GTCTGTACAGCCTGCAGTTGGCAAGCAGAGCCCTCCGACAGTGTGGAGCG
>N_read3
CTCGGAGCCTCATTGGTCTGTACAGCCTGCAGTTGGCAAGCAGAGCCCTC
>N_read4
AACTCTCTCCTGTACCTACTCAAATGCGTGAGGCCCTGTTGGAATGTATG
>N_read5
GCTCATTATAGGAAAAAACCACGCAGTAAATATTCGCAAAAGAAGCAAAC
>N_read6
GCGAGGCAGAACTCTCTCCTGTACCTACTCAAATGCGTGAGGCCCTGTTG
>N_read7
AAAAGCGTTTGAGAGACTAACAAAAATCTAATTATGAAGACAGCTCAGGC
>N_read8
CCGGGCGGCCAAACCCTGCAGCATCCAGGGCGGGCAGAGCCGCGCAGGTA
>N_read9
TCCCAGGGAGGACAGCCGGGCGGCCAAACCCTGCAGCATCCAGGGCGGGC
>N_read10
GGTGATGCGGATGTCCCCAGGTGCTGACTCGGGAGCACTGCGGGTCTCGG
>N_read11
AAAAGCGTTTGAGAGACTAACAAAAATCTAATTATGAAGACAGCTCAGGC
>N_read12
CCGCTGAGCTCCGAGGAGGGTGATGCGGATGTCCCCAGGTGCTGACTCGG
>N_read13
CCCTCTCTCTCTCTCTCTGTCTCCCAGCTCCACCTCTTACTACTCATGTG
>N_read14
CTACTCAAATGCGTGAGGCCCTGTTGGAATGTATGCAGGAGAAGAAAAAG
>N_read15
TCTTCGCCCGCTTCCCGCCAAGCATGGGATACTGCGAGGCAGAACTCTCT
>N_read16
AGGCGCTGCCGCGGAGCCTGGAGACAGTTAAGGACGCTGCACAGGTTCTT
>N_read17
GAGCTCCTATCTCGGCTTCCGCTGAGCTCCGAGGAGGGTGATGCGGATGT
>N_read18
GACAGTGTGGAGCGCGAAGATCGAAGAACACAGGGTCAGTTGCAGTGGAA
>N_read19
CTACTCATGTGACCTGGAATATTTGTAAAATGACAGTAATAGCAATATCT
>N_read20
CAGGGTCAGTTGCAGTGGAAGACGATTTATGCAGAAAGTCAGCTAGGACT
>N_read21
CAGCTAGGACTCTGCGCAGCTCCGTGTTCACCAGCCCAGGGTGCTGCTGA
>N_read22
TCCCAGGGAGGACAGCCGGGCGGCCAAACCCTGCAGCATCCAGGGCGGGC
>N_read23
CGGCTTCCGCTGAGCTCCGAGGAGGGTGATGCGGATGTCCCCAGGTGCTG
>N_read24
ACGCTGCACAGGTTCTTGTCTTCGCCCGCTTCCCGCCAAGCATGGGATAC
>N_read25
TGGAAGACGATTTATGCAGAAAGTCAGCTAGGACTCTGCGCAGCTCCGTG
>N_read26
GGCAAGGCTGCAAGCTCCCAGGGAGGACAGCCGGGCGGCCAAACCCTGCA
>N_read27
AAGACAGCTCAGGCTCATTATAGGAAAAAACCACGCAGTAAATATTCGCA
>N_read28
TGCAGGGGCAAGGCTGCAAGCTCCCAGGGAGGACAGCCGGGCGGCCAAAC
>N_read29
AGCACTGCGGGTCTCGGAGCCTCATTGGTCTGTACAGCCTGCAGTTGGCA
>N_read30
TCTCTCTCTCTCTCTGTCTCCCAGCTCCACCTCTTACTACTCATGTGACC
>N_read31
AGGCCCTGTTGGAATGTATGCAGGAGAAGAAAAAGCGTTTGAGAGACTAA
>N_read32
GGACTCTGCGCAGCTCCGTGTTCACCAGCCCAGGGTGCTGCTGAGAGCAG
>N_read33
TTGCAGTGGAAGACGATTTATGCAGAAAGTCAGCTAGGACTCTGCGCAGC
>N_read34
AAAAAACCACGCAGTAAATATTCGCAAAAGAAGCAAACAACAATGCTCAT
>N_read35
CCACGCAGTAAATATTCGCAAAAGAAGCAAACAACAATGCTCATTATTCC
>N_read36
CGGCTTCCGCTGAGCTCCGAGGAGGGTGATGCGGATGTCCCCAGGTGCTG
>N_read37
TCCCAGCTCCACCTCTTACTACTCATGTGACCTGGAATATTTGTAAAATG
>N_read38
CGCGGAGCCTGGAGACAGTTAAGGACGCTGCACAGGTTCTTGTCTTCGCC
>N_read39
ACGCCAGAGCTCCTATCTCGGCTTCCGCTGAGCTCCGAGGAGGGTGATGC
>N_read40
CCGGGCGGCCAAACCCTGCAGCATCCAGGGCGGGCAGAGCCGCGCAGGTA
>N_read41
CTGTACCTACTCAAATGCGTGAGGCCCTGTTGGAATGTATGCAGGAGAAG
>N_read42
CAGTTAAGGACGCTGCACAGGTTCTTGTCTTCGCCCGCTTCCCGCCAAGC
>N_read43
CCATATAATCAAAGAAATTTCTGCATCTTGCCACAGATCCTCCCTGATCT
>N_read44
GAGCGCGAAGATCGAAGAACACAGGGTCAGTTGCAGTGGAAGACGATTTA
>N_read45
CGCTGCCGCGGAGCCTGGAGACAGTTAAGGACGCTGCACAGGTTCTTGTC
>N_read46
GCCTCATTGGTCTGTACAGCCTGCAGTTGGCAAGCAGAGCCCTCCGACAG
>N_read47
CTCTGCGCAGCTCCGTGTTCACCAGCCCAGGGTGCTGCTGAGAGCAGGCG
>N_read48
AGTCAGCTAGGACTCTGCGCAGCTCCGTGTTCACCAGCCCAGGGTGCTGC
>N_read49
TCTCTCTCTGTCTCCCAGCTCCACCTCTTACTACTCATGTGACCTGGAAT
>N_read50
CCATATCAATTACTTCTCTCTCTCTCTCCCTCTCTCTCTCTCTCTGTCTC
>N_read51
CAGGCTCATTATAGGAAAAAACCACGCAGTAAATATTCGCAAAAGAAGCA
>N_read52
ATCTTGCCACAGATCCTCCCTGATCTTTTTCCATATCAATTACTTCTCTC
>N_read53
AAGAAGCAAACAACAATGCTCATTATTCCATATAATCAAAGAAATTTCTG
>N_read54
AGCGTTTGAGAGACTAACAAAAATCTAATTATGAAGACAGCTCAGGCTCA
>N_read55
GAGAGCAGGCGCTGCCGCGGAGCCTGGAGACAGTTAAGGACGCTGCACAG
>N_read56
ATCTTGCCACAGATCCTCCCTGATCTTTTTCCATATCAATTACTTCTCTC
>N_read57
TGCCGCGGAGCCTGGAGACAGTTAAGGACGCTGCACAGGTTCTTGTCTTC
>N_read58
GCGAGGCAGAACTCTCTCCTGTACCTACTCAAATGCGTGAGGCCCTGTTG
>N_read59
ACCCTGCAGCATCCAGGGCGGGCAGAGCCGCGCAGGTACCAGCAGGCTCG
>N_read60
CAGTGGAAGACGATTTATGCAGAAAGTCAGCTAGGACTCTGCGCAGCTCC
>N_read61
GCCCAGGGTGCTGCTGAGAGCAGGCGCTGCCGCGGAGCCTGGAGACAGTT
>N_read62
TTACTTCTCTCTCTCTCTCCCTCTCTCTCTCTCTCTGTCTCCCAGCTCCA
>N_read63
ATCCTCCCTGATCTTTTTCCATATCAATTACTTCTCTCTCTCTCTCCCTC
>N_read64
GCTGCAAGCTCCCAGGGAGGACAGCCGGGCGGCCAAACCCTGCAGCATCC
>N_read65
AATTTCTGCATCTTGCCACAGATCCTCCCTGATCTTTTTCCATATCAATT
>N_read66
GGAGGACAGCCGGGCGGCCAAACCCTGCAGCATCCAGGGCGGGCAGAGCC
>N_read67
CCCTCCGACAGTGTGGAGCGCGAAGATCGAAGAACACAGGGTCAGTTGCA
>N_read68
ACAGCCTGCAGTTGGCAAGCAGAGCCCTCCGACAGTGTGGAGCGCGAAGA
>N_read69
CAGTTGCAGTGGAAGACGATTTATGCAGAAAGTCAGCTAGGACTCTGCGC
>N_read70
TCTCTCTCTCCCTCTCTCTCTCTCTCTGTCTCCCAGCTCCACCTCTTACT
>N_read71
TGTTCACCAGCCCAGGGTGCTGCTGAGAGCAGGCGCTGCCGCGGAGCCTG
>N_read72
TTGCCACAGATCCTCCCTGATCTTTTTCCATATCAATTACTTCTCTCTCT
>N_read73
CTCATTATTCCATATAATCAAAGAAATTTCTGCATCTTGCCACAGATCCT
>N_read74
CTCTCTCTCTCTGTCTCCCAGCTCCACCTCTTACTACTCATGTGACCTGG
>N_read75
TATAGGAAAAAACCACGCAGTAAATATTCGCAAAAGAAGCAAACAACAAT
>N_read76
TGTACAGCCTGCAGTTGGCAAGCAGAGCCCTCCGACAGTGTGGAGCGCGA
>N_read77
GAAAGTCAGCTAGGACTCTGCGCAGCTCCGTGTTCACCAGCCCAGGGTGC
>N_read78
CTTTTTCCATATCAATTACTTCTCTCTCTCTCTCCCTCTCTCTCTCTCTC
>N_read79
AGAAGAAAAAGCGTTTGAGAGACTAACAAAAATCTAATTATGAAGACAGC
>N_read80
TCTCTCTCTCCCTCTCTCTCTCTCTCTGTCTCCCAGCTCCACCTCTTACT
>N_read81
GTATGCAGGAGAAGAAAAAGCGTTTGAGAGACTAACAAAAATCTAATTAT
>N_read82
GGTCTCGGAGCCTCATTGGTCTGTACAGCCTGCAGTTGGCAAGCAGAGCC
>N_read83
AGACAGTTAAGGACGCTGCACAGGTTCTTGTCTTCGCCCGCTTCCCGCCA
>N_read84
CTCTCTCTCTCTGTCTCCCAGCTCCACCTCTTACTACTCATGTGACCTGG
>N_read85
GCTGCAAGCTCCCAGGGAGGACAGCCGGGCGGCCAAACCCTGCAGCATCC
>N_read86
GTATGCAGGAGAAGAAAAAGCGTTTGAGAGACTAACAAAAATCTAATTAT
>N_read87
GCTGCAAGCTCCCAGGGAGGACAGCCGGGCGGCCAAACCCTGCAGCATCC
>N_read88
CTCCTATCTCGGCTTCCGCTGAGCTCCGAGGAGGGTGATGCGGATGTCCC
>N_read89
GAGCGCGAAGATCGAAGAACACAGGGTCAGTTGCAGTGGAAGACGATTTA
>N_read90
CTCTCTCCCTCTCTCTCTCTCTCTGTCTCCCAGCTCCACCTCTTACTACT
>N_read91
TCCGACAGTGTGGAGCGCGAAGATCGAAGAACACAGGGTCAGTTGCAGTG
>N_read92
TATCAATTACTTCTCTCTCTCTCTCCCTCTCTCTCTCTCTCTGTCTCCCA
>N_read93
CGCTGCCGCGGAGCCTGGAGACAGTTAAGGACGCTGCACAGGTTCTTGTC
>N_read94
AATGTATGCAGGAGAAGAAAAAGCGTTTGAGAGACTAACAAAAATCTAAT
>N_read95
TGGAGACAGTTAAGGACGCTGCACAGGTTCTTGTCTTCGCCCGCTTCCCG
>N_read96
CCAGGGCGGGCAGAGCCGCGCAGGTACCAGCAGGCTCGAACGCCAGAGCT
>N_read97
ACGCTGCACAGGTTCTTGTCTTCGCCCGCTTCCCGCCAAGCATGGGATAC
>N_read98
TTGCAGTGGAAGACGATTTATGCAGAAAGTCAGCTAGGACTCTGCGCAGC
>N_read99
GAAAGTCAGCTAGGACTCTGCGCAGCTCCGTGTTCACCAGCCCAGGGTGC
>N_read100
CTCATTATTCCATATAATCAAAGAAATTTCTGCATCTTGCCACAGATCCT
>E_read1
CTCTCTCAATTTCTCTACGGTGGGGAACACCTAAGCAAATCGTCAGCTTT
>E_read2
AAAACAGATGACCAAACTGCTGGATATTTCATGGCAAGTTCCCATAGCCA
>E_read3
TTGACATTTGTAATTATGATCGCTGTTATTATCATTGTTAGGAGAGTAAC
>E_read4
TTTGTAATTATGATCGCTGTTATTATCATTGTTAGGAGAGTAACGAGTAA
>E_read5
AAATGAAACAAAACAGATGACCAAACTGCTGGATATTTCATGGCAAGTTC
>E_read6
CGTTCTTCCTTTAGAGTTTCCCTTCCTCCTGTTTCCTACCCAACTTGTTC
>E_read7
CCAACTTGTTCTCTCTCTCAATTTCTCTACGGTGGGGAACACCTAAGCAA
>E_read8
ATTATCATTGTTAGGAGAGTAACGAGTAACAAATGAAACAAAACAGATGA
>E_read9
TGTTCTCTCTCTCAATTTCTCTACGGTGGGGAACACCTAAGCAAATCGTC
>E_read10
AGTTCCCATAGCCAACTCTGTCGCATCCGTTCTTCCTTTAGAGTTTCCCT
>E_read11
CATTATTGCACTTGCAACACCTAGCACTTGACATTTGTAATTATGATCGC
>E_read12
AGCAAATCGTCAGCTTTATGCATCACTGTTCACAGAGGAGTGGGAGACAA
>E_read13
AAATGAAACAAAACAGATGACCAAACTGCTGGATATTTCATGGCAAGTTC
>E_read14
TTTGTAATTATGATCGCTGTTATTATCATTGTTAGGAGAGTAACGAGTAA
>E_read15
CACTTGACATTTGTAATTATGATCGCTGTTATTATCATTGTTAGGAGAGT
>E_read16
TATTTCATGGCAAGTTCCCATAGCCAACTCTGTCGCATCCGTTCTTCCTT
>E_read17
GGATATTTCATGGCAAGTTCCCATAGCCAACTCTGTCGCATCCGTTCTTC
>E_read18
CTTCCTCCTGTTTCCTACCCAACTTGTTCTCTCTCTCAATTTCTCTACGG
>E_read19
GGTGGGGAACACCTAAGCAAATCGTCAGCTTTATGCATCACTGTTCACAG
>E_read20
GGTGGGGAACACCTAAGCAAATCGTCAGCTTTATGCATCACTGTTCACAG
>E_read21
GGATATTTCATGGCAAGTTCCCATAGCCAACTCTGTCGCATCCGTTCTTC
>E_read22
AGGAGAGTAACGAGTAACAAATGAAACAAAACAGATGACCAAACTGCTGG
>E_read23
GTTAGGAGAGTAACGAGTAACAAATGAAACAAAACAGATGACCAAACTGC
>E_read24
TCCCTTCCTCCTGTTTCCTACCCAACTTGTTCTCTCTCTCAATTTCTCTA
>E_read25
CCTGTTTCCTACCCAACTTGTTCTCTCTCTCAATTTCTCTACGGTGGGGA
>E_read26
TGTTCTCTCTCTCAATTTCTCTACGGTGGGGAACACCTAAGCAAATCGTC
>E_read27
ATGATCGCTGTTATTATCATTGTTAGGAGAGTAACGAGTAACAAATGAAA
>E_read28
TCCTACCCAACTTGTTCTCTCTCTCAATTTCTCTACGGTGGGGAACACCT
>E_read29
AAAACAGATGACCAAACTGCTGGATATTTCATGGCAAGTTCCCATAGCCA
>E_read30
GGTGGGGAACACCTAAGCAAATCGTCAGCTTTATGCATCACTGTTCACAG
>E_read31
TCTCTCTCTCAATTTCTCTACGGTGGGGAACACCTAAGCAAATCGTCAGC
>E_read32
ATCATTGTTAGGAGAGTAACGAGTAACAAATGAAACAAAACAGATGACCA
>E_read33
GTAATTATGATCGCTGTTATTATCATTGTTAGGAGAGTAACGAGTAACAA
>E_read34
ATTATGATCGCTGTTATTATCATTGTTAGGAGAGTAACGAGTAACAAATG
>E_read35
AGGAGAGTAACGAGTAACAAATGAAACAAAACAGATGACCAAACTGCTGG
>E_read36
AAAACAGATGACCAAACTGCTGGATATTTCATGGCAAGTTCCCATAGCCA
>E_read37
GCTGGATATTTCATGGCAAGTTCCCATAGCCAACTCTGTCGCATCCGTTC
>E_read38
AGAGTAACGAGTAACAAATGAAACAAAACAGATGACCAAACTGCTGGATA
>E_read39
TTCACAGAGGAGTGGGAGACAATTGTATCCTACTCTTTCCTCCTCTCAGA
>E_read40
AACAAATGAAACAAAACAGATGACCAAACTGCTGGATATTTCATGGCAAG
>E_read41
ATGGCAAGTTCCCATAGCCAACTCTGTCGCATCCGTTCTTCCTTTAGAGT
>E_read42
ATGATCGCTGTTATTATCATTGTTAGGAGAGTAACGAGTAACAAATGAAA
>E_read43
ACAGATGACCAAACTGCTGGATATTTCATGGCAAGTTCCCATAGCCAACT
>E_read44
ACTGCTGGATATTTCATGGCAAGTTCCCATAGCCAACTCTGTCGCATCCG
>E_read45
GCTGGATATTTCATGGCAAGTTCCCATAGCCAACTCTGTCGCATCCGTTC
>E_read46
GTAATTATGATCGCTGTTATTATCATTGTTAGGAGAGTAACGAGTAACAA
>E_read47
CTCTGTCGCATCCGTTCTTCCTTTAGAGTTTCCCTTCCTCCTGTTTCCTA
>E_read48
CGCATCCGTTCTTCCTTTAGAGTTTCCCTTCCTCCTGTTTCCTACCCAAC
>E_read49
ATTGTTAGGAGAGTAACGAGTAACAAATGAAACAAAACAGATGACCAAAC
>E_read50
ACAGATGACCAAACTGCTGGATATTTCATGGCAAGTTCCCATAGCCAACT
>E_read51
CTCTACGGTGGGGAACACCTAAGCAAATCGTCAGCTTTATGCATCACTGT
>E_read52
AGCCAACTCTGTCGCATCCGTTCTTCCTTTAGAGTTTCCCTTCCTCCTGT
>E_read53
TTTAGAGTTTCCCTTCCTCCTGTTTCCTACCCAACTTGTTCTCTCTCTCA
>E_read54
GCTGGATATTTCATGGCAAGTTCCCATAGCCAACTCTGTCGCATCCGTTC
>E_read55
TGAAACAAAACAGATGACCAAACTGCTGGATATTTCATGGCAAGTTCCCA
>E_read56
TCCCATAGCCAACTCTGTCGCATCCGTTCTTCCTTTAGAGTTTCCCTTCC
>E_read57
TTTCTCTACGGTGGGGAACACCTAAGCAAATCGTCAGCTTTATGCATCAC
>E_read58
AGTAACAAATGAAACAAAACAGATGACCAAACTGCTGGATATTTCATGGC
>E_read59
AACAAATGAAACAAAACAGATGACCAAACTGCTGGATATTTCATGGCAAG
>E_read60
AACAAAACAGATGACCAAACTGCTGGATATTTCATGGCAAGTTCCCATAG
>E_read61
GTTAGGAGAGTAACGAGTAACAAATGAAACAAAACAGATGACCAAACTGC
>E_read62
AGCCAACTCTGTCGCATCCGTTCTTCCTTTAGAGTTTCCCTTCCTCCTGT
>E_read63
TCTTCCTTTAGAGTTTCCCTTCCTCCTGTTTCCTACCCAACTTGTTCTCT
>E_read64
CATTATTGCACTTGCAACACCTAGCACTTGACATTTGTAATTATGATCGC
>E_read65
TAGCACTTGACATTTGTAATTATGATCGCTGTTATTATCATTGTTAGGAG
>E_read66
GGGGAACACCTAAGCAAATCGTCAGCTTTATGCATCACTGTTCACAGAGG
>E_read67
ATTATCATTGTTAGGAGAGTAACGAGTAACAAATGAAACAAAACAGATGA
>E_read68
AGTAACAAATGAAACAAAACAGATGACCAAACTGCTGGATATTTCATGGC
>E_read69
TCTCAATTTCTCTACGGTGGGGAACACCTAAGCAAATCGTCAGCTTTATG
>E_read70
CCAACTTGTTCTCTCTCTCAATTTCTCTACGGTGGGGAACACCTAAGCAA
>E_read71
TTCATGGCAAGTTCCCATAGCCAACTCTGTCGCATCCGTTCTTCCTTTAG
>E_read72
AACACCTAGCACTTGACATTTGTAATTATGATCGCTGTTATTATCATTGT
>E_read73
CATAGCCAACTCTGTCGCATCCGTTCTTCCTTTAGAGTTTCCCTTCCTCC
>E_read74
ACTTGCAACACCTAGCACTTGACATTTGTAATTATGATCGCTGTTATTAT
>E_read75
TCTTCCTTTAGAGTTTCCCTTCCTCCTGTTTCCTACCCAACTTGTTCTCT
>E_read76
CAAACTGCTGGATATTTCATGGCAAGTTCCCATAGCCAACTCTGTCGCAT
>E_read77
AGAGTAACGAGTAACAAATGAAACAAAACAGATGACCAAACTGCTGGATA
>E_read78
ACTGTTCACAGAGGAGTGGGAGACAATTGTATCCTACTCTTTCCTCCTCT
>E_read79
TCAGCTTTATGCATCACTGTTCACAGAGGAGTGGGAGACAATTGTATCCT
>E_read80
ATTATCATTGTTAGGAGAGTAACGAGTAACAAATGAAACAAAACAGATGA
>E_read81
AACAAAACAGATGACCAAACTGCTGGATATTTCATGGCAAGTTCCCATAG
>E_read82
TTATGCATCACTGTTCACAGAGGAGTGGGAGACAATTGTATCCTACTCTT
>E_read83
GATGACCAAACTGCTGGATATTTCATGGCAAGTTCCCATAGCCAACTCTG
>E_read84
TGCATCACTGTTCACAGAGGAGTGGGAGACAATTGTATCCTACTCTTTCC
>E_read85
ACTGCTGGATATTTCATGGCAAGTTCCCATAGCCAACTCTGTCGCATCCG
>E_read86
GTAACGAGTAACAAATGAAACAAAACAGATGACCAAACTGCTGGATATTT
>E_read87
GATGACCAAACTGCTGGATATTTCATGGCAAGTTCCCATAGCCAACTCTG
>E_read88
TATTTCATGGCAAGTTCCCATAGCCAACTCTGTCGCATCCGTTCTTCCTT
>E_read89
ATCGCTGTTATTATCATTGTTAGGAGAGTAACGAGTAACAAATGAAACAA
>E_read90
TCTCAATTTCTCTACGGTGGGGAACACCTAAGCAAATCGTCAGCTTTATG
>E_read91
TCAGCTTTATGCATCACTGTTCACAGAGGAGTGGGAGACAATTGTATCCT
>E_read92
TTTCTCTACGGTGGGGAACACCTAAGCAAATCGTCAGCTTTATGCATCAC
>E_read93
TTTCTCTACGGTGGGGAACACCTAAGCAAATCGTCAGCTTTATGCATCAC
>E_read94
TGTCGCATCCGTTCTTCCTTTAGAGTTTCCCTTCCTCCTGTTTCCTACCC
>E_read95
CATTATTGCACTTGCAACACCTAGCACTTGACATTTGTAATTATGATCGC
>E_read96
TGTTCTCTCTCTCAATTTCTCTACGGTGGGGAACACCTAAGCAAATCGTC
>E_read97
ATGATCGCTGTTATTATCATTGTTAGGAGAGTAACGAGTAACAAATGAAA
>E_read98
AGAGTTTCCCTTCCTCCTGTTTCCTACCCAACTTGTTCTCTCTCTCAATT
>E_read99
GTTATTATCATTGTTAGGAGAGTAACGAGTAACAAATGAAACAAAACAGA
>E_read100
ATCATTGTTAGGAGAGTAACGAGTAACAAATGAAACAAAACAGATGACCA
